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Sangon Biotech negative control sirna si scramble
Negative Control Sirna Si Scramble, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of HOTAIR on gastric cancer cell apoptosis, migration and invasion in vitro . BGC-823 cells were transfected with si-HOTAIR or si-NC, and SGC-7901 cells were transfected with pCDNA/HOTAIR vector or empty vector control. (A) Hoechst staining assay of cell apoptosis; the percentage of Hoechst-positive nuclei per optical field (at least 50 fields) was determined. (B) The apoptotic rates of cells were detected by flow cytometry. UL, necrotic cells; UR, terminal apoptotic cells; LR, early apoptotic cells. (C and D) Transwell assays were performed to investigate changes in cell migration and invasiveness. *P < 0.05 and **P < 0.01.

Journal: Molecular Cancer

Article Title: Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer

doi: 10.1186/1476-4598-13-92

Figure Lengend Snippet: The effect of HOTAIR on gastric cancer cell apoptosis, migration and invasion in vitro . BGC-823 cells were transfected with si-HOTAIR or si-NC, and SGC-7901 cells were transfected with pCDNA/HOTAIR vector or empty vector control. (A) Hoechst staining assay of cell apoptosis; the percentage of Hoechst-positive nuclei per optical field (at least 50 fields) was determined. (B) The apoptotic rates of cells were detected by flow cytometry. UL, necrotic cells; UR, terminal apoptotic cells; LR, early apoptotic cells. (C and D) Transwell assays were performed to investigate changes in cell migration and invasiveness. *P < 0.05 and **P < 0.01.

Article Snippet: Three individual HOTAIR siRNAs (si-HOTAIR) and scrambled negative control siRNA (si-NC) were purchased from Invitrogen (Invitrogen, CA, USA).

Techniques: Migration, In Vitro, Transfection, Plasmid Preparation, Staining, Flow Cytometry